Recombinant S. Japonicum Glutathione S-transferase/GST protein

GST, also known as, Glutathione S-transferase, represents a major group of detoxification enzymes. GST acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur) . The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. GST was originally separated from Schistosomajaponicum but currently isolated from recombinant E. coli source. Recombinant Schistosoma japonicum GST was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 30 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Product Name Alternative

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

Expression System

E.coli

Antigen Species

Schistosoma japonicum

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 30unit/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Molecular Weight

25.4 kDa (218aa)

Additionnal Information

Glutathione S-Transferase, GST, Glutathione S-Transferase, Sj26 antigen, ATGP3792-10 µg, ATGP3792-20 µg, ATGP3792-50 µg, ATGP3792-100 µg, ATGP3792-250 µg, ATGP3792-500 µg, ATGP3792-1 mg, ATGP3792-10, ATGP3792-20, ATGP3792-50, ATGP3792-100, ATGP3792-250, ATGP3792-500, ATGP3792-1

References & Citations

Hayes JD., et al. (1995) Crit Rev BiochemMol Biol. 30 (6) :445-600.; ; Smith DB., et al. (1988) Mol Biochem Parasitol. 27 (2-3) :249-56.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Fusion Tag & Detection

NCBI Accession Number

AAB59203

Uniprot Accession Number

P08515

Species

Schistosoma japonicum

AA Sequence

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPK