Recombinant human FGF-21 (L174P) protein

FGF-21 is a unique member of FGF super family that is reported to be primarily produced by the liver. FGF-21 has recently been shown to have beneficial effects on a variety of metabolic parameters, including glucose and lipid metabolism and insulin sensitivity in animal models and is thought to exert potent antidiabetic and lipid-lowering agent of obesity and type 2 diabetes mellitus. FGF-21 activity depends on membrane protein beta Klotho that physically complexes with various FGF receptors, thus conferring them the ability to bind FGF-21 and activate downstream signaling pathways. Recombinant human FGF-21 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Fibroblast growth factor 21

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

21.6 kDa (202aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

FGF21 (L174P), Fibroblast growth factor 21, ATGP3789-10 µg, ATGP3789-20 µg, ATGP3789-50 µg, ATGP3789-100 µg, ATGP3789-250 µg, ATGP3789-500 µg, ATGP3789-1 mg, ATGP3789-10, ATGP3789-20, ATGP3789-50, ATGP3789-100, ATGP3789-250, ATGP3789-500, ATGP3789-1

References & Citations

Dostalova I., et al. (2009) Physiol Res. 58 (1) :1-7; ; Kharitonenkov A., et al. (2008) BioDrugs. 22 (1) :37-44.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_061986

Uniprot Accession Number

Q9NSA1

Species

Human

AA Sequence

MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVK TSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPAPPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS