Recombinant human FGF acidic/FGF-1 protein

Acidic fibroblast growth factor (aFGF) belongs to the fibroblast growth factor family. It is a potent mitogenic agent. aFGF has been detected in large amounts in the brain. Other cells known to express FGF acidic include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. The growth factor acts through activation of specific cell-surface receptors leading to intracellular tyrosine phosphorylation cascade. Recombinant human aFGF was overexpressed in E. coli and purified by conventional chromatography.

Product Specifications

Product Name Alternative

Acidic Fibroblast Growth Factor, Fibroblast growth factor 1, Acidic fibroblast growth factor, aFGF, Endothelial cell growth factor, ECGF, Heparin-binding growth factor 1, HBGF-1, FGFA, ECGFA, ECGF-beta, FGF-alpha, GLIO703, ECGFB

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

15.9 kDa (141aa) confirmed by MALDI-TOF

Additionnal Information

FGF acidic, Acidic Fibroblast Growth Factor, ATGP3788-10 µg, ATGP3788-20 µg, ATGP3788-50 µg, ATGP3788-100 µg, ATGP3788-250 µg, ATGP3788-500 µg, ATGP3788-1 mg, ATGP3788-10, ATGP3788-20, ATGP3788-50, ATGP3788-100, ATGP3788-250, ATGP3788-500, ATGP3788-1

References & Citations

Jaye M. et al., (1986) Science. 233, 541-544.; ; Mergia A. et al., (1989) Biochem. Biophys. Res. Commun. 164, 1121-1129.; ; Stauber DJ. et al., (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 49-54.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.0) containing 100 mM NaCl

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_000791

Uniprot Accession Number

P05230

Species

Human

AA Sequence

MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D