Recombinant human CD3 delta/CD3D protein
CD3D, also known as T-cell surface glycoprotein CD3 delta chain isoform A, is a single-pass type 1 membrane protein. This protein together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor (TCR) alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. Also, this protein plays an essential role in adaptive immune response and plays an essential role in thymocyte differentiation. Recombinant human CD3D, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
36.5 kDa (323aa)
Additionnal Information
References & Citations
Hori T., et al, (1991) Int. Immunol. 3:353-357.; ; Phillips JH., et al, (1992) J. Exp. Med. 175:1055-1066.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_000723
Uniprot Accession Number
P04234
Species
Human
N Terminal Sequence
Phe22
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