Recombinant human Ephrin-A1 protein

EFNA1, also known as ephrin-A1 isoform a, is a member of the Ephrin-A family of GPI-anchored ligands. The Ephrin family receptor interacting proteins serve as the ligands of the Ephrin receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases. This protein contains on ephrin RBD (ephrin receptor-binding) domain. It is widely expressed on endothelial and epithelial a cells, particularly in the lung, intestine, liver, and skin. Also, this protein is expressed on resting CD4+ T cells inhibits cell proliferation and activation although soluble protein can promote T cell chemotaxis. Recombinant human EFNA1, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human EPHA2 (CAT# ATGP3378) is coated at 2 µg/mL (100 ul/well) can bind Human Ephrin-A1. The ED50 range ≤ 50 ng/mL.

Product Name Alternative

Ephrin A1, EFNA1, EPH-related receptor tyrosine kinase ligand 1, LERK-1, Immediate early response protein B61, Tumor necrosis factor alpha-induced protein 4, TNF alpha-induced protein 4, gastric cancer metastasis associated long noncoding RNA, EPLG1, LERK1, TNFAIP4, ECKLG, GMAN

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human EPHA2 (CAT# ATGP3378) . The ED50 range ≤ 50 ng/mL.

Molecular Weight

46.6 kDa (406aa)

Additionnal Information

Ephrin-A1, Ephrin-A1 isoform a, EFNA1, B61, ECKLG, EFL1, EPLG1, LERK1, TNFAIP4, ATGP3782-10 µg, ATGP3782-20 µg, ATGP3782-50 µg, ATGP3782-100 µg, ATGP3782-250 µg, ATGP3782-500 µg, ATGP3782-1 mg, ATGP3782-10, ATGP3782-20, ATGP3782-50, ATGP3782-100, ATGP3782-250, ATGP3782-500, ATGP3782-1

References & Citations

Beckmann MP., et al, (1994) EMBO J. 13:3757-3762.; ; Hasmim M., et al, (2013) BMC Cancer 13:592-604.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_004419.1

Uniprot Accession Number

P20827

Species

Human

N Terminal Sequence

Ala

AA Sequence

DRHTVFW NSSNPKFRNE DYTIHVQLND YVDIICPHYE DHSVADAAME QYILYLVEHE EYQLCQPQSK DQVRWQCNRP SAKHGPEKLS EKFQRFTPFT LGKEFKEGHS YYYISKPIHQ HEDRCLRLKV TVSGKITHSP QAHVNPQEKR LAADDPEVRV LHSIGHS