Recombinant human IL-5R alpha/IL5RA protein

IL5RA, also known as interleukin-5 receptor subunit alpha, is a subunit of the Interleukin-5 receptor. The alpha chain binds to IL5. It generates soluble secreted forms which function as IL-5 antagonists and promotes the differentiation of basophils and B cells. This protein plays also a roll of T-cell-derived cytokine which is important in the development of asthma for the lerminal differentiation, activation and survival of committed cosinophil precursors. Recombinant human IL5RA protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Interleukin-5 receptor subunit alpha isoform 1, IL5RA, IL-5R subunit alpha, IL5R, IL-5 receptor subunit alpha, HSIL5R3, CDw125, CD125

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

37.8 kDa (331aa)

Additionnal Information

Interleukin-5 receptor subunit alpha isoform 1, Interleukin 5 receptor subunit alpha isoform 1, IL5RA, IL-5R subunit alpha, IL5R subunit alpha, IL5R, IL-5 receptor subunit alpha, IL5 receptor subunit alpha, IL5 R a, IL 5R subunit alpha, IL 5 receptor subunit alpha, HSIL5R3, CDw125, CD125, CD 125, ATGP3781-10 µg, ATGP3781-20 µg, ATGP3781-50 µg, ATGP3781-100 µg, ATGP3781-250 µg, ATGP3781-500 µg, ATGP3781-1 mg, ATGP3781-010, ATGP3781-020, ATGP3781-050, ATGP3781-100, ATGP3781-250, ATGP3781-500, ATGP3781-01M

References & Citations

Khorasanizadeh M., et al, (2016) Int Rev Immunol. 35:294-311.; ; Van Rensburg IC, et al, (2017) Oncotarget. 8:2037-2043.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_000555

Uniprot Accession Number

Q01344

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPDLLPDEK ISLLPPVNFT IKVTGLAQVL LQWKPNPDQE QRNVNLEYQV KINAPKEDDY ETRITESKCV TILHKGFSAS VRTILQNDHS LLASSWASAE LHAPPGSPGT SIVNLTCTTN TTEDNYSRLR SYQVSLHCTW LVGTDAPEDT QYFLYYRYGS WTEECQEYSK DTLGRNIACW FPRTFILSKG RDWLAVLVNG SSKHSAIRPF DQLFALHAID QINPPLNVTA EIEGTRLSIQ WEKPVSAFPI HCFDYEVKIH NTRNGYLQIE KLMTNAFISI IDDLSKYDVQ VRAAVSSMCR EAGLWSEWSQ PIYVGNDEHK PLREWHHHHH H