Recombinant human Hemopexin protein

HPX, also known as hemopexin, is a plasma glycoprotein that belongs to the family of the acute-phase proteins whose synthesis is induced after an inflammatory event. This protein associates with HDL and influence the inflammatory properties of HDL. In addition it can also be said that HDL from HX-null mice is pro-inflammatory. Also, this protein binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation and is expressed in the central nervous system, in the retina, and in peripheral nerves has also been observed. Recombinant human HPX, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Hemopexin, HPX, HX

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

50.4 kDa (448aa)

Additionnal Information

Hemopexin, Hemopexin, HPX, HX, ATGP3780-10 µg, ATGP3780-20 µg, ATGP3780-50 µg, ATGP3780-100 µg, ATGP3780-250 µg, ATGP3780-500 µg, ATGP3780-1 mg, ATGP3780-10, ATGP3780-20, ATGP3780-50, ATGP3780-100, ATGP3780-250, ATGP3780-500, ATGP3780-1

References & Citations

Takahashi N., et al, (1985) Proc. Natl. Acad. Sci. U.S.A. 82:73-77.; ; Mauk MR., et al, (2007) Nat Prod Rep. 24:523-532.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000604

Uniprot Accession Number

P02790

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPTPLPPTS AHGNVAEGET KPDPDVTERC SDGWSFDATT LDDNGTMLFF KGEFVWKSHK WDRELISERW KNFPSPVDAA FRQGHNSVFL IKGDKVWVYP PEKKEKGYPK LLQDEFPGIP SPLDAAVECH RGECQAEGVL FFQGDREWFW DLATGTMKER SWPAVGNCSS ALRWLGRYYC FQGNQFLRFD PVRGEVPPRY PRDVRDYFMP CPGRGHGHRN GTGHGNSTHH GPEYMRCSPH LVLSALTSDN HGATYAFSGT HYWRLDTSRD GWHSWPIAHQ WPQGPSAVDA AFSWEEKLYL VQGTQVYVFL TKGGYTLVSG YPKRLEKEVG TPHGIILDSV DAAFICPGSS RLHIMAGRRL WWLDLKSGAQ ATWTELPWPH EKVDGALCME KSLGPNSCSA NGPGLYLIHG PNLYCYSDVE KLNAAKALPQ PQNVTSLLGC THHHHHHH