Recombinant human MEK1 protein

MAP2K1, is a member of the bispecific protein kinase family, which acts as a mitogen-activated protein (MAP) kinase. It is expressed extensively and brain levels are very low. It is located upstream of the MAP kinase and stimulates the enzymatic activity of MAP kinases on a variety of extra-and-cellular signals. This protein, an essential component of the MAP kinase signaling pathway, is involved in many cellular processes such as proliferation, differentiation, transcriptional regulation and development. Recombinant human MAP2K1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Dual specificity mitogen-activated protein kinase kinase 1, MAP2K1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

44.5 kDa (402aa)

Additionnal Information

MEK1, Dual specificity mitogen-activated protein kinase kinase 1, MAP2K1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1, ATGP3779-10 µg, ATGP3779-20 µg, ATGP3779-50 µg, ATGP3779-100 µg, ATGP3779-250 µg, ATGP3779-500 µg, ATGP3779-1 mg, ATGP3779-10, ATGP3779-20, ATGP3779-50, ATGP3779-100, ATGP3779-250, ATGP3779-500, ATGP3779-1

References & Citations

Hirata H., et al. (2012) Carcinogenesis. 33:41-48.; ; Rampoldi L., et al. (1998) Cytogenet Cell Genet. 78:301-303.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002746.1

Uniprot Accession Number

Q02750

Species

Human

N Terminal Sequence

Ala

AA Sequence

MPKKKPT PIQLNPAPDG SAVNGTSSAE TNLEALQKKL EELELDEQQR KRLEAFLTQK QKVGELKDDD FEKISELGAG NGGVVFKVSH KPSGLVMARK LIHLEIKPAI RNQIIRELQV LHECNSPYIV GFYGAFYSDG EISICMEHMD GGSLDQVLKK AGRIPEQILG KVSIAVIKGL TYLREKHKIM HRDVKPSNIL VNSRGEIKLC DFGVSGQLID SMANSFVGTR SYMSPERLQG THYSVQSDIW SMGLSLVEMA VGRYPIPPPD AKELELMFGC QVEGDAAETP PRPRTPGRPL SSYGMDSRPP MAIFELLDYI VNEPPPKLPS GVFSLEFQDF VNKCLIKNPA ERADLKQLMV HAFIKRSDAE EVDFAGWLCS TIGLNQPSTP THAAGV