Recombinant mouse Prolactin protein

Prolactin, also known as prl, is a hormone synthesized and secreted by lacto trope cells in the adenohypophysis (anterior pituitary gland) . Prolactin has many effects, the most significant of which is to stimulate the mammary glands to produce milk (lactation) . Increased serum prolactin during pregnancy causes enlargement of the mammary glands of the breasts and increases the production of milk. Recently, prolactin has demonstrated broader roles in breast cancer development, regulation of reproductive function, and immuno-regulation. Recombinant mouse Prolactin, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Prolactin isoform 1, AV290867, Gha1, Prl1a1, Growth hormone a1

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

25 kDa (222aa) Confirmed by MALDI-TOF

Additionnal Information

Prolactin, Prolactin isoform 1, AV290867, Gha1, Prl1a1, Growth hormone a1, ATGP3777-10 µg, ATGP3777-20 µg, ATGP3777-50 µg, ATGP3777-100 µg, ATGP3777-250 µg, ATGP3777-500 µg, ATGP3777-1 mg, ATGP3777-10, ATGP3777-20, ATGP3777-50, ATGP3777-100, ATGP3777-250, ATGP3777-500, ATGP3777-1

References & Citations

Suzuki MF., et al. (2012) Biotechnol Appl Biochem. 59 (3) :178-85; ; Goffin V., et al. (1999) Mol Cell Endocrinol. 151 (1-2) :79-87.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_035294

Uniprot Accession Number

Q9CPQ2

Species

Mouse

AA Sequence

QPLPICS AGDCQTSLRE LFDRVVILSH YIHTLYTDMF IEFDKQYVQD REFMVKVIND CPTSSLATPE DKEQALKVPP EVLLNLILSL VQSSSDPLFQ LITGVGGIQE APEYILSRAK EIEEQNKQLL EGVEKIISQA YPEAKGNGIY FVWSQLPSLQ GVDEESKILS LRNTIRCLRR DSHKVDNFLK VLRCQIAHQN NC