Recombinant human Biglycan protein
BGN, also known as biglycan preproprotein, is a small leucine-rich repeat proteoglycan (SLRP) . It plays an important role in the development and maintenance of many tissues. Core protein of this protein binds to the growth factors BMP-4 and influences its bioactivity. This protein is also involved in collagen fiber assembly. Recombinant human BGN protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human Biglycan in inhibit the cell growth using 3T3-L1 mouse embryonic fibroblast adipose-like cells. The ED50 range ≤ 20 µg/mL.
Product Name Alternative
Biglycan preproprotein, BGN, DSPG1, MRLS, PG-S1, PGI, SEMDX, SLRR1A
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in inhibit the cell growth using 3T3-L1 mouse embryonic fibroblast adipose-like cells. The ED50 range ≤ 20 µg/mL.
Molecular Weight
38.3 kDa (340aa)
Additionnal Information
References & Citations
Sun H., et al, (2016) Arch Gynecol Obstet. 293:429-438.; ; Gaspar R., et al, (2016) J Mol Cell Cardiol. 99:138-150.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_001702.1
Uniprot Accession Number
P21810
Species
Human
N Terminal Sequence
Ala
AA Sequence
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