Recombinant mouse Biglycan protein

Bgn, also known as biglycan preproprotein, is a small leucine-rich repeat proteoglycan (SLRP) . It binds to the growth factors BMP-4 and influences BMP-4 bioactivity. It plays an important role in stabilizing fibrotic scars after experimental myocardial infarction. It may function in connective tissue metabolism by binding to collagen fibrils and TGF-beta and may promote neuronal survival. Its ablation improves cardiac function and attenuates left ventricular hypertrophy and fibrosis after long-term pressure overload. It is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. Recombinant mouse Bgn, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Biglycan, Bgn, BG, DSPG1, PG-S1, PGI, SLRR1A

Expression System

Baculovirus

Antigen Species

Mouse

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

64.6 kDa (574aa)

Additionnal Information

Biglycan, Biglycan, Bgn, BG, DSPG1, PG-S1, PGI, SLRR1A, ATGP3775-10 µg, ATGP3775-20 µg, ATGP3775-50 µg, ATGP3775-100 µg, ATGP3775-250 µg, ATGP3775-500 µg, ATGP3775-1 mg, ATGP3775-10, ATGP3775-20, ATGP3775-50, ATGP3775-100, ATGP3775-250, ATGP3775-500, ATGP3775-1

References & Citations

Beetz N., et al. (2016) J Mol Cell Cardiol. 101:145-155.; ; Bianco P., et al. (1990) J Histochem Cytochem. 38:1549-1563.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_031568

Uniprot Accession Number

P28653

Species

Mouse

N Terminal Sequence

Ala

AA Sequence

ADPDEEASGS DTTSGVPDLD SVTPTFSAMC PFGCHCHLRV VQCSDLGLKT VPKEISPDTT LLDLQNNDIS ELRKDDFKGL QHLYALVLVN NKISKIHEKA FSPLRKLQKL YISKNHLVEI PPNLPSSLVE LRIHDNRIRK VPKGVFSGLR NMNCIEMGGN PLENSGFEPG AFDGLKLNYL RISEAKLTGI PKDLPETLNE LHLDHNKIQA IELEDLLRYS KLYRLGLGHN QIRMIENGSL SFLPTLRELH LDNNKLSRVP AGLPDLKLLQ VVYLHSNNIT KVGINDFCPM GFGVKRAYYN GISLFNNPVP YWEVQPATFR CVTDRLAIQF GNYKKLEPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHH