Recombinant human Granzyme B protein
GZMB, also known as granzyme B isoform 1, is member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. Tis protein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. Also, it processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. Recombinant human GZMB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.
Product Name Alternative
Granzyme B isoform 1, GZMB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 2,500pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.
Molecular Weight
26.5 kDa (235aa)
Additionnal Information
References & Citations
Poe M., et al, (1991) J. Biol. Chem. 266:98-103.; ; Xu W., et al, (2014) Eur. J. Immunol. 44 (1) 275-284.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT
Scientific Category
Apoptosis
NCBI Accession Number
NP_004122.2
Uniprot Accession Number
P10144
Species
Human
N Terminal Sequence
Gly19
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