Recombinant human SDF2 protein

SDF2, also known as stromal cell-derived factor 2, is believed to be a secretory protein. The amino acid sequence deduced from the murine clone and the human homolog are conserved more than 92%, and the aa sequence of SDF2 shows similarity to those of yeast dolichyl phosphate-D-mannose, protein mannosyltransferases. Its expression is ubiquitous and the gene appears to be relatively conserved among mammals. Also, this protein is involved to stromal derived factors with SDF-1, SDF-4 and SDF-5. These are a loosely defined group of molecules that are generated by stromal cells. SDF group have prognostic value and warrant further investigation in their biological functions and clinical value. Recombinant human SDF2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Stromal cell-derived factor 2, SDF2, SDF-2

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.3 kDa (202aa)

Additionnal Information

SDF2, Stromal cell-derived factor 2, SDF2, SDF-2, ATGP3773-10 µg, ATGP3773-20 µg, ATGP3773-50 µg, ATGP3773-100 µg, ATGP3773-250 µg, ATGP3773-500 µg, ATGP3773-1 mg, ATGP3773-10, ATGP3773-20, ATGP3773-50, ATGP3773-100, ATGP3773-250, ATGP3773-500, ATGP3773-1

References & Citations

Hamada T., et al, (1996) Gene 176:211-214.; ; Kang H., et al, (2009) Int. J. Oncol. 35:205-211.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 50 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl, 0.1mM PMSF, 0.5mM EDTA

Scientific Category

Signal Transduction

NCBI Accession Number

NP_008854

Uniprot Accession Number

Q99470

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPSSLGVVT CGSVVKLLNT RHNVRLHSHD VRYGSGSGQQ SVTGVTSVDD SNSYWRIRGK SATVCERGTP IKCGQPIRLT HVNTGRNLHS HHFTSPLSGN QEVSAFGEEG EGDYLDDWTV LCNGPYWVRD GEVRFKHSST EVLLSVTGEQ YGRPISGQKE VHGMAQPSQN NYWKAMEGIF MKPSELLKAE AHHAELHHHH HH