Recombinant human SDF2 protein
SDF2, also known as stromal cell-derived factor 2, is believed to be a secretory protein. The amino acid sequence deduced from the murine clone and the human homolog are conserved more than 92%, and the aa sequence of SDF2 shows similarity to those of yeast dolichyl phosphate-D-mannose, protein mannosyltransferases. Its expression is ubiquitous and the gene appears to be relatively conserved among mammals. Also, this protein is involved to stromal derived factors with SDF-1, SDF-4 and SDF-5. These are a loosely defined group of molecules that are generated by stromal cells. SDF group have prognostic value and warrant further investigation in their biological functions and clinical value. Recombinant human SDF2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Stromal cell-derived factor 2, SDF2, SDF-2
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
22.3 kDa (202aa)
Additionnal Information
References & Citations
Hamada T., et al, (1996) Gene 176:211-214.; ; Kang H., et al, (2009) Int. J. Oncol. 35:205-211.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 50 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl, 0.1mM PMSF, 0.5mM EDTA
Scientific Category
Signal Transduction
NCBI Accession Number
NP_008854
Uniprot Accession Number
Q99470
Species
Human
N Terminal Sequence
Ala
AA Sequence
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