Recombinant human NFATC2 protein

NFATC2, also known as nuclear factor of activated T-cells cytoplasmic 2 isoform C, is a member of the nuclear factor of activated T cells family. This protein plays a vital role in the course of T helper cell activation, differentiation, and effector function. Although KO of an individual NFAT isoform in T cells leads to rather mild effects, T cells deficient for NFATC1 and 2 completely fail to produce T helper cell effector cytokines, such as the interleukins IL-2 and IL-4 or interferon gamma. Also, it is highly phosphorylated and retained in the cytoplasm. Following T cell receptor (TCR) stimulation, dephosphorylation by calcium-activated calcineurin induces a conformational change of NFATC2 that exposes one or more nuclear localization sequences. Recombinant human NFATC2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.