Recombinant human MGAT2 protein

MGAT2, also known as alpha-1, 6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, is a single-pass type 2 membrane protein. It has the typical glycosyltransferase domains that composes a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. This protein catalyzes an essential step in the conversion of oligo-mannose to complex N-glycans. Sometimes mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. Recombinant human MGAT2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, Mannosyl alpha-1,6-glycoprotein beta-1,2-N-acetylglucosaminyltransferas, GlcNAc-T II, GNT-II, Mannoside acetylglucosaminyltransferase 2, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II, CDG2A, CDGS2

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

49.3 kDa (427aa)

Additionnal Information

MGAT2, Alpha-1, 6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, MGAT2, CDG2A, CDGS2, GLCNACTII, GNT-II, GNT2, ATGP3770-10 µg, ATGP3770-20 µg, ATGP3770-50 µg, ATGP3770-100 µg, ATGP3770-250 µg, ATGP3770-500 µg, ATGP3770-1 mg, ATGP3770-10, ATGP3770-20, ATGP3770-50, ATGP3770-100, ATGP3770-250, ATGP3770-500, ATGP3770-1

References & Citations

Tan J., et al, (1996) Am. J. Hum. Genet. 59:810-817.; ; Maszczak-Seneczko D., et al, (2015) J. Biol. Chem. 290:15475-15486.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol, 0.15M NaCl, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_002399

Uniprot Accession Number

Q10469

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPRQRKNEA LAPPLLDAEP ARGAGGRGGD HPSVAVGIRR VSNVSAASLV PAVPQPEADN LTLRYRSLVY QLNFDQTLRN VDKAGTWAPR ELVLVVQVHN RPEYLRLLLD SLRKAQGIDN VLVIFSHDFW STEINQLIAG VNFCPVLQVF FPFSIQLYPN EFPGSDPRDC PRDLPKNAAL KLGCINAEYP DSFGHYREAK FSQTKHHWWW KLHFVWERVK ILRDYAGLIL FLEEDHYLAP DFYHVFKKMW KLKQQECPEC DVLSLGTYSA SRSFYGMADK VDVKTWKSTE HNMGLALTRN AYQKLIECTD TFCTYDDYNW DWTLQYLTVS CLPKFWKVLV PQIPRIFHAG DCGMHHKKTC RPSTQSAQIE SLLNNNKQYM FPETLTISEK FTVVAISPPR KNGGWGDIRD HELCKSYRRL QHHHHHH