Recombinant human CD36/SR-B3 protein

CD36, also known as platelet glycoprotein 4 isoform 1, is an integral membrane glycoprotein. This protein is found on the surface of cells that is thought to have a 'hairpin-like' structure with alpha-helices at the C- and N- termini projecting through the membrane and a larger extracellular loop. It is broadly expressed on a variety of cell types including adipocytes, skeletal muscle, and epithelial cells of the retina, breast, intestine, and megakaryocytes. Also, it is required for the anti-angiogenic effects of thrombospondin-1 in the corneal neovascularization assay. It plays a role in lipid metabolism and plays a significant role in the initiation and pathogenesis of chronic inflammatory diseases such as Alzheimer s disease and atherosclerosis. Recombinant human CD36, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Platelet glycoprotein 4 isoform 1, CD36, BDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, SCARB3

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Molecular Weight

73.9 kDa (652aa)

Additionnal Information

CD36, SR-B3, Platelet glycoprotein 4 isoform 1, CD36, BDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, SCARB3, ATGP3768-10 µg, ATGP3768-20 µg, ATGP3768-50 µg, ATGP3768-100 µg, ATGP3768-250 µg, ATGP3768-500 µg, ATGP3768-1 mg, ATGP3768-10, ATGP3768-20, ATGP3768-50, ATGP3768-100, ATGP3768-250, ATGP3768-500, ATGP3768-1

References & Citations

Tandon NN., et al, (1989) J. Biol. Chem. 264:7570-7575; ; Wei P., et al, (2017) J. Biol. Chem. 292:8683-8693.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_000063

Uniprot Accession Number

P16671

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADLGDLLIQK TIKKQVVLEE GTIAFKNWVK TGTEVYRQFW IFDVQNPQEV MMNSSNIQVK QRGPYTYRVR FLAKENVTQD AEDNTVSFLQ PNGAIFEPSL SVGTEADNFT VLNLAVAAAS HIYQNQFVQM ILNSLINKSK SSMFQVRTLR ELLWGYRDPF LSLVPYPVTT TVGLFYPYNN TADGVYKVFN GKDNISKVAI IDTYKGKRNL SYWESHCDMI NGTDAASFPP FVEKSQVLQF FSSDICRSIY AVFESDVNLK GIPVYRFVLP SKAFASPVEN PDNYCFCTEK IISKNCTSYG VLDISKCKEG RPVYISLPHF LYASPDVSEP IDGLNPNEEE HRTYLDIEPI TGFTLQFAKR LQVNLLVKPS EKIQVLKNLK RNYIVPILWL NETGTIGDEK ANMFRSQVTG KINLEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH