Recombinant human PCPE-1 protein

PCOLCE, also known as procollagen C-endopeptidase enhancer 1, is a secreted extracellular matrix glycoprotein. This protein is expressed primarily by interstitial connective tissues such as tendons, calvaria, and skin. Also, fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. Also, this protein binds and drives the enzymatic cleavage of type 1 procollagen and heightens C-proteinase activity. Recombinant human PCOLCE, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Procollagen C-endopeptidase enhancer 1, PCOLCE PCPE, PCPE-1, PCPE1

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

46.6 kDa (433aa)

Additionnal Information

PCPE1, Procollagen C-endopeptidase enhancer 1, PCOLCE PCPE, PCPE-1, PCPE1, ATGP3765-10 µg, ATGP3765-20 µg, ATGP3765-50 µg, ATGP3765-100 µg, ATGP3765-250 µg, ATGP3765-500 µg, ATGP3765-1 mg, ATGP3765-10, ATGP3765-20, ATGP3765-50, ATGP3765-100, ATGP3765-250, ATGP3765-500, ATGP3765-1

References & Citations

Takahara K., et al, (1994) J. Biol. Chem. 269:26280-26285.; ; Scott IC., et al, (1999) Genomics 55:229-234.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002584

Uniprot Accession Number

Q15113

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPQTPNYTR PVFLCGGDVK GESGYVASEG FPNLYPPNKE CIWTITVPEG QTVSLSFRVF DLELHPACRY DALEVFAGSG TSGQRLGRFC GTFRPAPLVA PGNQVTLRMT TDEGTGGRGF LLWYSGRATS GTEHQFCGGR LEKAQGTLTT PNWPESDYPP GISCSWHIIA PPDQVIALTF EKFDLEPDTY CRYDSVSVFN GAVSDDSRRL GKFCGDAVPG SISSEGNELL VQFVSDLSVT ADGFSASYKT LPRGTAKEGQ GPGPKRGTEP KVKLPPKSQP PEKTEESPSA PDAPTCPKQC RRTGTLQSNF CASSLVVTAT VKSMVREPGE GLAVTVSLIG AYKTGGLDLP SPPTGASLKF YVPCKQCPPM KKGVSYLLMG QVEENRGPVL PPESFVVLHR PNQDQILTNL SKRKCPSQPV RAAASQDHHH HHH