Recombinant human TLR3 protein
TLR3, also known as toll-like receptor 3, is a member of the toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. It is innate immune receptors for sensing microbial molecules and damage-associated molecular patterns released from host cells. It recognizes dsRNA and activation of the receptor induces the activation of NF-kappaB and the production of type I interferons (IFNs) . Its signaling activation is associated with ischemic preconditioning-induced protection against brain ischemia and attenuation of reactive astrogliosis. It is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. Recombinant human TLR3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Toll-like receptor 3, TLR3, CD283, IIAE2
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
78.5 kDa (690aa)
Additionnal Information
References & Citations
Alexopoulou L., et al. (2001) Nature. 413:732-738.; ; Cole JE., et al. (2011) Proc Natl Acad Sci U S A. 108:2372-2377.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 20% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_003256
Uniprot Accession Number
O15455
Species
Human
N Terminal Sequence
Ser23
AA Sequence
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