Recombinant mouse HPD protein

Hpd, also known as 4-hydroxyphenylpyruvate dioxygenase, is a Fe-containing enzyme that catalyzes the second reaction in the catabolism of tyrosine the conversion of 4-hydroxyphenylpyruvate to homogentisate. Existing as a homodimer, Hpd uses zinc as a cofactor to catalyze the third step in the conversion of L-phenylalanine to fumarate and acetoacetic acid. Defects in the gene encoding Hpd are the cause of tyrosinemia type 3 and hawkinsinuria, both of which are inborn errors of metabolism that are associated with a variety of symptoms, including mental retardation and seizures and hair and urine abnormalities. Recombinant mouse Hpd protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

4-hydroxyphenylpyruvate dioxygenase, 4HPPD, Fla, Flp, Hppd, Laf, 4-hydroxyphenylpyruvic acid oxidase, HPPDase, F Alloantigen, F protein

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Molecular Weight

47.4 kDa (416aa) confirmed by MALDI-TOF

Additionnal Information

Hpd, 4-hydroxyphenylpyruvate dioxygenase, 4HPPD, Fla, Flp, Hppd, Laf, ATGP3760-10 µg, ATGP3760-20 µg, ATGP3760-50 µg, ATGP3760-100 µg, ATGP3760-250 µg, ATGP3760-500 µg, ATGP3760-1 mg, ATGP3760-10, ATGP3760-20, ATGP3760-50, ATGP3760-100, ATGP3760-250, ATGP3760-500, ATGP3760-1

References & Citations

Wada GH., et al. (1975) J Biol Chem J. 250 (17) :6720-6.; ; Endo F., et al. (1997) J Biol Chem. 26; ;272 (39) :24426-32.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_032303

Uniprot Accession Number

P49429

Species

Mouse

AA Sequence

MTTYNNK GPKPERGRFL HFHSVTFWVG NAKQAASFYC NKMGFEPLAY RGLETGSREV VSHVIKQGKI VFVLCSALNP WNKEMGDHLV KHGDGVKDIA FEVEDCDHIV QKARERGAKI VREPWVEQDK FGKVKFAVLQ TYGDTTHTLV EKINYTGRFL PGFEAPTYKD TLLPKLPRCN LEIIDHIVGN QPDQEMQSAS EWYLKNLQFH RFWSVDDTQV HTEYSSLRSI VVTNYEESIK MPINEPAPGR KKSQIQEYVD YNGGAGVQHI ALKTEDIITA IRHLRERGTE FLAAPSSYYK LLRENLKSAK IQVKESMDVL EELHILVDYD EKGYLLQIFT KPMQDRPTLF LEVIQRHNHQ GFGAGNFNSL FKAFEEEQAL RGNLTDLEPN GVRSGM