Recombinant human CD52 protein

CD52, also known as CAMPATH-1 antigen, is a small glycosylphosphatidylinositol (GPI) anchored glycoprotein that is tethered to the cell surface with a GPI linkage. This protein can be targeted to induce immune suppression by complement-meditated cell lysis. CD52 is expressed on lymphocytes, monocytes, monocytes-derived dendritic cells, eosinophils, and neutrophils, but not on the stem cells from which these lymphocytes were derived. Also, it is an important target for therapeutic interventions aimed at leukocyte depletion in hematological malignancies and post-transplant immunosuppression. Recombinant human CD52, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CAMPATH-1 antigen, CD52, CDW52, EDDM5

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

28.2 kDa (251aa)

Additionnal Information

CD52, CAMPATH-1 antigen, CD52, CDW52, EDDM5, ATGP3748-10 µg, ATGP3748-20 µg, ATGP3748-50 µg, ATGP3748-100 µg, ATGP3748-250 µg, ATGP3748-500 µg, ATGP3748-1 mg, ATGP3748-10, ATGP3748-20, ATGP3748-50, ATGP3748-100, ATGP3748-250, ATGP3748-500, ATGP3748-1

References & Citations

Xia MQ., et al, (1991) Eur. J. Immunol. 21:1677-1684.; ; Rowan WC., et al, (1995) Int. Immunol. 7:69-77.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_001794

Uniprot Accession Number

P31358

Species

Human

N Terminal Sequence

Gly25

AA Sequence

GQNDTSQTSS PSLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H