Recombinant human Protein C Inhibitor/SERPINA5 protein
Serpin A5, also known as PCI (Protein C Inhibitor) or PAI3 (plasminogen activator inhibitor 3), is a member of the serpin serine proteinase inhibitor family. This protein inhibits plasminogen activators as well as activated protein C. Serpin A5 is secreted in plasma, but is also expressed in liver. Serpin A5 is involved in cell inflammation, proliferation, apoptosis, tumor cell migration, invasion, and metastasis. Recombinant Serpin A5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Measured by its ability to inhibit Thrombin cleavage of substrate Boc-VPR-AMC. The IC50 for this effect is less or equal to 2 nM
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its ability to inhibit Thrombin cleavage of substrate Boc-VPR-AMC. The IC50 for this effect is less or equal to 2 nM
Molecular Weight
45.9 kDa (408aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Han MH., et al. (2008) Nature. 451 (7182) :1076-81. ; ; Li W., et al. (2008) J Biol Chem. 283 (51) :36039-45.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM MES buffer (pH 6.0) containing 150 mM NaCl, 10% glycerol
Scientific Category
Cardiovascular
NCBI Accession Number
AAH08915
Uniprot Accession Number
P05154
Species
Human
AA Sequence
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
MUC18 / Mucin 18 / CD146 (OJ79c), CF647 conjugate, 0.1mg/mL
CD59 (heavy chain of Protectin) (BRA-10G), CF647 conjugate, 0.1mg/mL
CD50 / ICAM3 (CG106), CF594 conjugate, 0.1mg/mL
