Recombinant human HLA-G protein

HLA-G, also known as HLA class I histocompatibility antigen, alpha chain G, belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin) . The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. HLA-G is a non-classical class-I HLA molecule associated with anti-inflammatory and immuno-modulatory properties which interacts with inhibitory receptors (ILT2/ILT4/KIR2DL4) present on various immune cells. It inhibits proliferation of B cells, T cells and natural killer cells; and also induces regulatory T cells. Recombinant human HLA-G, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

HLA class I histocompatibility antigen, alpha chain G, MHC-G

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Molecular Weight

35.3 kDa (309aa) confirmed by MALDI-TOF

Additionnal Information

HLA-G, ATGP3743-10 µg, ATGP3743-20 µg, ATGP3743-50 µg, ATGP3743-100 µg, ATGP3743-250 µg, ATGP3743-500 µg, ATGP3743-1 mg, ATGP3743-10, ATGP3743-20, ATGP3743-50, ATGP3743-100, ATGP3743-250, ATGP3743-500, ATGP3743-1

References & Citations

Poomarimuthu M., et al. (2017) Pediatr Rheumatol Online J. 15 (1) :10.; ; Sanders SK., et al. (1991) J Exp Med. 174 (3) :737-40.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_002118.1

Uniprot Accession Number

P17693

Species

Human

AA Sequence

GSHSM RYFSAAVSRP GRGEPRFIAM GYVDDTQFVR FDSDSACPRM EPRAPWVEQE GPEYWEEETR NTKAHAQTDR MNLQTLRGYY NQSEASSHTL QWMIGCDLGS DGRLLRGYEQ YAYDGKDYLA LNEDLRSWTA ADTAAQISKR KCEAANVAEQ RRAYLEGTCV EWLHRYLENG KEMLQRADPP KTHVTHHPVF DYEATLRCWA LGFYPAEIIL TWQRDGEDQT QDVELVETRP AGDGTFQKWA AVVVPSGEEQ RYTCHVQHEG LPEPLMLRWK QSSLPTIPI