Recombinant mouse Hemoglobin zeta/HBZ protein

Hba-x, also known as Hemoglobin subunit zeta (Hbz), is belongs to the globin family. Zeta-globin is alpha-like hemoglobin. The Hba-x polypeptide is synthesized in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal and adult life. Hba-x gene includes five functional genes and two pseudogenes. The order of genes is: 5' -zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 -alpha-1 - theta1 - 3'. Recombinant mouse Hba-x fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Hemoglobin subunit zeta, Alpha-like embryonic globin chain x, Hemoglobin zeta chain, Zeta-globin, HBZ1, HBZ-T1, Hba-x

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Molecular Weight

18.3 kDa (162aa) confirmed by MALDI-TOF

Additionnal Information

Hba-x, Hemoglobin subunit zeta, AI450015, Hbz, ATGP3740-10 µg, ATGP3740-20 µg, ATGP3740-50 µg, ATGP3740-100 µg, ATGP3740-250 µg, ATGP3740-500 µg, ATGP3740-1 mg, ATGP3740-10, ATGP3740-20, ATGP3740-50, ATGP3740-100, ATGP3740-250, ATGP3740-500, ATGP3740-1

References & Citations

Lau ET., et al. (2001) Prenat. Diagn. 21 (7) : 529-39; ; Kidd RD., et al. (2001) Biochemistry. 40 (51) :15669-75

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM MES buffer (pH 6.0) containing 0.1M NaCl, 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_034535

Uniprot Accession Number

P06467

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSLMKNERAI IMSMWEKMAA QAEPIGTETL ERLFCSYPQT KTYFPHFDLH HGSQQLRAHG FKIMTAVGDA VKSIDNLSSA LTKLSELHAY ILRVDPVNFK LLSHCLLVTM AARFPADFTP EVHEAWDKFM SILSSILTEK YR