Recombinant mouse IL-12 p70 protein

IL12b/IL12a, also known as interleukin 12 (subunit beta/alpha), is a cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. It is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. As it is associated with IL23A to form the IL-23 interleukin, it induces autoimmune inflammation and is responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. Recombinant mouse IL12b/IL12a protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Mouse IL12 stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range is less or equal to 10 ng/mL.

Product Name Alternative

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 for this effect is less or equal to 10 ng/mL.

Molecular Weight

35.7 kDa (p40, 313aa) ; ; 22.5 kDa (p35, 199aa) ; ; The heterodimeric Il12 has a predicted Molecular weight of 58.3 kDa (512aa) .; ; Molecular weight on SDS-PAGE will appear higher

Additionnal Information

IL12A, IL12B, CLMF p35, CLMF p40, Cytotoxic lymphocyte maturation factor 35 kDa subunit, Cytotoxic lymphocyte maturation factor 40 kDa subunit, Interleukin-12 alpha chain, Interleukin-12 beta chain, NK cell stimulatory factor chain 1, p35, NK cell stimulatory factor chain 2, p40, NKSF1, NKSF2, IL12, IL-12, Interleukin12, Interleukin 12, IL 12, Interleukin 12 alpha chain, Interleukin 12 beta chain, ATGP3739-10 µg, ATGP3739-20 µg, ATGP3739-50 µg, ATGP3739-100 µg, ATGP3739-250 µg, ATGP3739-500 µg, ATGP3739-1 mg, ATGP3739-010, ATGP3739-020, ATGP3739-050, ATGP3739-100, ATGP3739-250, ATGP3739-500, ATGP3739-01M