Recombinant rat TNF-alpha protein

Tnf also known as Tumor necrosis factor. The Protein acts as a cytokine binds TNF receptors plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response. Recombinant rat Tnf, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Rat TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Product Name Alternative

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

Expression System

E.coli

Antigen Species

Rat

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2 ng/mL.

Molecular Weight

19.9 kDa (181aa) confirmed by MALDI-TOF

Additionnal Information

TNF-a, ATGP3736-10 µg, ATGP3736-20 µg, ATGP3736-50 µg, ATGP3736-100 µg, ATGP3736-250 µg, ATGP3736-500 µg, ATGP3736-1 mg, ATGP3736-10, ATGP3736-20, ATGP3736-50, ATGP3736-100, ATGP3736-250, ATGP3736-500, ATGP3736-1

References & Citations

Furuya T., et al. (2001) Genes Immun. 2:229-232.; ; Warle M.C., et al. (2005) Transpl. Immunol. 14:77-82.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_036807

Uniprot Accession Number

P16599

Species

Rat

AA Sequence

LRSSS QNSSDKPVAH VVANHQAEEQ LEWLSQRANA LLANGMDLKD NQLVVPADGL YLIYSQVLFK GQGCPDYVLL THTVSRFAIS YQEKVSLLSA IKSPCPKDTP EGAELKPWYE PMYLGGVFQL EKGDLLSAEV NLPKYLDITE SGQVYFGVIA L