Recombinant human IL-1R beta/IL1R2 protein
IL1R2, also known as interleukin-1 receptor type 2 isoform 1, is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein is expressed predominantly by, T cells, fibroblasts and endothelial cells. It binds interleukin-1 alpha (IL1A), interleukin-1beta (IL1B), and interleukin 1 receptor antagonist (IL1RN), preventing them from binding to their regular receptors and thereby inhibiting the transduction of their signaling. Also, Interleukin-4 (IL4) is reported to antagonize the activity of interleukin-1 by inducing the expression and release of this cytokine. Recombinant human IL1R2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 range ≤400 ng/mL with Human IL-1 beta/IL-1F2.
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 range ≤400 ng/mL with Human IL-1 beta/IL-1F2.
Molecular Weight
38.8 kDa (338aa)
Additionnal Information
References & Citations
Greenfeder SA., et al, (1995) J Biol Chem 270:13757-13765.; ; Granowitz EV., et al, (1991) J. Biol. Chem. 266:14147-14150.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_004624.1
Uniprot Accession Number
P27930
Species
Human
N Terminal Sequence
Phe14
Available Sizes
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