Recombinant human ART4 protein

ART4, also known as ecto-ADP-ribosyltransferase 4, is a glycosylphosphatidylinositol (GPI) -linked membrane protein carrying Dombrock blood group antigens. It is developmentally regulated during erythroid differentiation and occurs at highest levels in the fetal liver. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. Recombinant human ART4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ecto-ADP-ribosyltransferase 4 isoform 1, ART4, ARTC4, CD297, DO, DOK1

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Molecular Weight

28.8 kDa (248aa)

Additionnal Information

ART4, Ecto-ADP-ribosyltransferase 4 isoform 1, ART4, ARTC4, CD297, DO, DOK1, ATGP3731-10 µg, ATGP3731-20 µg, ATGP3731-50 µg, ATGP3731-100 µg, ATGP3731-250 µg, ATGP3731-500 µg, ATGP3731-1 mg, ATGP3731-10, ATGP3731-20, ATGP3731-50, ATGP3731-100, ATGP3731-250, ATGP3731-500, ATGP3731-1

References & Citations

Grodecka M., et al. (2012) Immunohematology. 28:124-129.; ; Gubin AN., et al. (2000) Blood. 96:2621-2627.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_066549

Uniprot Accession Number

Q93070

Species

Human

N Terminal Sequence

Ala

AA Sequence

SEVAIKI DFDFAPGSFD DQYQGCSKQV MEKLTQGDYF TKDIEAQKNY FRMWQKAHLA WLNQGKVLPQ NMTTTHAVAI LFYTLNSNVH SDFTRAMASV ARTPQQYERS FHFKYLHYYL TSAIQLLRKD SIMENGTLCY EVHYRTKDVH FNAYTGATIR FGQFLSTSLL KEEAQEFGNQ TLFTIFTCLG APVQYFSLKK EVLIPPYELF KVINMSYHPR GDWLQLRSTG NLSTYNCQLL KA