Recombinant mouse EGF protein
Egf, also known as Pro-epidermal growth factor isoform1, is a 8. 6 kDa globular protein containing 77 amino acids residues, including 3 intra-molecular disulfide bonds. Egf is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, Egf has been shown to inhibit gastric secretion, and to be involved in wound healing. Egf signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant mouse Egf protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional column chromatography
Product Specifications
Specifications
Mouse EGF in a cell proliferation assay using mouse Balb/3T3 cell. The ED50 range ≤ 1 ng/mL.
Product Name Alternative
Epidermal growth factor, Pro-epidermal growth factor isoform1, AI790464
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using mouse Balb/3T3 cell. The ED50 range ≤ 1 ng/mL.
Molecular Weight
8.6 kDa (77aa) Confirmed by MALDI-TOF
Additionnal Information
References & Citations
Riese., et al (1998) Bioessays. 20 (1) :41-8.; ; Cohen S (1983) Cancer. 51 (10) :1787-91.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 2mM DTT, 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_034243
Uniprot Accession Number
P01132
Species
Mouse
AA Sequence
NSYPGC PSSYDGYCLN GGVCMHIESL DSYTCNCVIG YSGDRCQTRD LRWWELR
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