Recombinant human Chromogranin A/CHGA protein

CHGA, also known as chromogranin-A isoform 1 preproprotein, is a member of neuroendocrine secretory proteins of the chromogranin / secretogranin family. It is a precursor of several functional peptides including vasostatin-1, vasostatin-2, pancreastatin, catestatin and parastatin. This peptide negatively regulates the neuroendocrine function of autocrine or nearby cells (paracrine) . This protein induces and promotes the production of secretory granules containing insulin in pancreatic islet beta cells. Recombinant human CHGA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cga, Pituitary secretory protein I, SP-I, Vasostatin-1, Vasostatin-2, EA-92, ES-43, Pancreastatin, parastatin, PHE5, PHES, SS-18, WA-8, WE-14, LF-19, Catestatin 2, SL21, AL-11, GV-19, GR-44, ER-37, GE-25, Serpinin-RRG, Serpinin, p-Glu serpinin precursor

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Molecular Weight

50kDa (448aa)

Additionnal Information

Vasostatin2, Vasostatin 2, Vasostatin, SP-I, SPI, SP-1, SP1, SP I, SP 1, Pituitary secretory protein I, Parathyroid secretory protein 1, Parastatin, Pancreastatin, Chromogranin-A isoform 1, Chromogranin-A, ChromograninA, Chromogranin A precursor, Chromogranin A parathyroid secretory protein 1, Chromogranin A isoform 1, Chromogranin A, CHGA, CHG A, CGA, beta Granin, Chromogranin-A precursor, Chromogranin-A parathyroid secretory protein 1, ATGP3728-10 µg, ATGP3728-20 µg, ATGP3728-50 µg, ATGP3728-100 µg, ATGP3728-250 µg, ATGP3728-500 µg, ATGP3728-1 mg, ATGP3728-010, ATGP3728-020, ATGP3728-050, ATGP3728-100, ATGP3728-250, ATGP3728-500, ATGP3728-01M

References & Citations

Nikoopour E., et al. (2011) J Immunol. 186:3831-3835.; ; Stadinski BD., et al. (2010) Nat Immunol. 11:225-231.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 0.1mM PMSF

Scientific Category

Neuroscience

NCBI Accession Number

NP_001266.1

Uniprot Accession Number

P10645

Species

Human

N Terminal Sequence

Ala

AA Sequence

LPVNSPM NKGDTEVMKC IVEVISDTLS KPSPMPVSQE CFETLRGDER ILSILRHQNL LKELQDLALQ GAKERAHQQK KHSGFEDELS EVLENQSSQA ELKEAVEEPS SKDVMEKRED SKEAEKSGEA TDGARPQALP EPMQESKAEG NNQAPGEEEE EEEEATNTHP PASLPSQKYP GPQAEGDSEG LSQGLVDREK GLSAEPGWQA KREEEEEEEE EAEAGEEAVP EEEGPTVVLN PHPSLGYKEI RKGESRSEAL AVDGAGKPGA EEAQDPEGKG EQEHSQQKEE EEEMAVVPQG LFRGGKSGEL EQEEERLSKE WEDSKRWSKM DQLAKELTAE KRLEGQEEEE DNRDSSMKLS FRARAYGFRG PGPQLRRGWR PSSREDSLEA GLPLQVRGYP EEKKEEEGSA NRRPEDQELE SLSAIEAELE KVAHQLQALR RG