Recombinant human Histamine N-Methyltransferase/HNMT protein

HNMT also known as Histamine N-methyltransferase. HNMT is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. HNMT inactivates histamine by N-methylation. Histamine is involved in regulation and modulation of immune response through the stimulation of four distinct subtypes of receptors, H1, H2, H3, and H4, present on the target cells. Histamine is inactivated by the histamine-metabolizing enzyme histamine N-methyltransferase (HNMT) in bronchus, kidney, and the central nervous system. It plays an important role in degrading histamine and in regulating the airway response to histamine. Recombinant human HNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is > 200 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C

Product Name Alternative

Histamine N-methyltransferase isoform 1, HMT, HNMT-S1, HNMT-S2, MRT51

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 200 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C

Molecular Weight

37.4 kDa (328aa) confirmed by MALDI-TOF

Additionnal Information

Histamine N-Methyltransferase, HNMT, Histamine N-methyltransferase isoform 1, HMT, HNMT-S1, HNMT-S2, MRT51, ATGP3727-10 µg, ATGP3727-20 µg, ATGP3727-50 µg, ATGP3727-100 µg, ATGP3727-250 µg, ATGP3727-500 µg, ATGP3727-1 mg, ATGP3727-10, ATGP3727-20, ATGP3727-50, ATGP3727-100, ATGP3727-250, ATGP3727-500, ATGP3727-1

References & Citations

Garcia-Martin E., et al. (2009) Pharmacogenomics. 10 (5) :867-83.; ; Palikhe NS., et al. (2008) J Clin Pharm Ther. 33 (5) :465-72.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

AAH20677

Uniprot Accession Number

P50135

Species

Human

AA Sequence

MASS MRSLFSDHGK YVESFRRFLN HSTEHQCMQE FMDKKLPGII GRIGDTKSEI KILSIGGGAG EIDLQILSKV QAQYPGVCIN NEVVEPSAEQ IAKYKELVAK TSNLENVKFA WHKETSSEYQ SRMLEKKELQ KWDFIHMIQM LYYVKDIPAT LKFFHSLLGT NAKMLIIVVS GSSGWDKLWK KYGSRFPQDD LCQYITSDDL TQMLDNLGLK YECYDLLSTM DISDCFIDGD ENGDLLWDFL TETCNFNATA PPDLRAELGK DLQEPEFSAK KEGKVLFNNT LSFIVIEA