Recombinant human IFN-gamma/IFNG protein
IFNG, also known as Interferon gamma, is secreted by lymphocytes stimulated by mitogen and is involved in the differentiation, maturation, and proliferation of hematopoietic cells. Nature human interferon-gamma is composed of 143 amino acid residues without cysteine residues and is glycosylated. Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. Recombinant human IFNG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human IFN-gamma stimulates cytotoxicity of the WiDr human colon colorectal adenocarcinoma cells. The ED50 range ≤5 ng/mL.
Product Name Alternative
Interferon gamma, Immune interferon, IFG, IFI, IMD69
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cytotoxicity assay using WiDr human colon colorectal adenocarcinoma cells. The ED50 range ≤5 ng/mL.
Molecular Weight
18.4 kDa (159aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Arakawa T., et al. (1987) Biochemistry. 26 (17) :5428-32; ; Rinderknecht E., et al. (1984) J Biol Chem. 259 (11) :6790-7
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_000610
Uniprot Accession Number
P01579
Species
Human
AA Sequence
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