Recombinant human IFN-gamma/IFNG protein

IFNG, also known as Interferon gamma, is secreted by lymphocytes stimulated by mitogen and is involved in the differentiation, maturation, and proliferation of hematopoietic cells. Nature human interferon-gamma is composed of 143 amino acid residues without cysteine residues and is glycosylated. Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. Recombinant human IFNG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human IFN-gamma stimulates cytotoxicity of the WiDr human colon colorectal adenocarcinoma cells. The ED50 range ≤5 ng/mL.

Product Name Alternative

Interferon gamma, Immune interferon, IFG, IFI, IMD69

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cytotoxicity assay using WiDr human colon colorectal adenocarcinoma cells. The ED50 range ≤5 ng/mL.

Molecular Weight

18.4 kDa (159aa) confirmed by MALDI-TOF

Additionnal Information

IFN-g, ATGP3723-10 µg, ATGP3723-20 µg, ATGP3723-50 µg, ATGP3723-100 µg, ATGP3723-250 µg, ATGP3723-500 µg, ATGP3723-1 mg, ATGP3723-10, ATGP3723-20, ATGP3723-50, ATGP3723-100, ATGP3723-250, ATGP3723-500, ATGP3723-1

References & Citations

Arakawa T., et al. (1987) Biochemistry. 26 (17) :5428-32; ; Rinderknecht E., et al. (1984) J Biol Chem. 259 (11) :6790-7

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_000610

Uniprot Accession Number

P01579

Species

Human

AA Sequence

QDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRG