Recombinant mouse Malate dehydrogenase 2/MDH2 protein

Mdh2, also known as Malate dehydrogenase 2. Mdh2 catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, Mdh2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. It is widely expressed with high expression levels found in adrenal, small intestine, heart and pancreas. Recombinant mouse Mdh2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 800 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C

Product Name Alternative

Malate dehydrogenase, mitochondrial, MDH, Mdh-2, Mor-1, Mor1

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 800unit/mg, and is defined as the amount of enzyme that cleaves 1umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C

Molecular Weight

35.4 kDa (335aa) confirmed by MALDI-TOF

Additionnal Information

Mdh2, Malate dehydrogenase, mitochondrial, MDH, Mdh-2, Mor-1, Mor1, ATGP3722-10 µg, ATGP3722-20 µg, ATGP3722-50 µg, ATGP3722-100 µg, ATGP3722-250 µg, ATGP3722-500 µg, ATGP3722-1 mg, ATGP3722-10, ATGP3722-20, ATGP3722-50, ATGP3722-100, ATGP3722-250, ATGP3722-500, ATGP3722-1

References & Citations

Wang L., et al. (2010) Dev Dyn. 239 (3) :954-64; ; Chang AK., et al. (2002) J Basic Microbiol. 42 (4) :238-45.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_032643

Uniprot Accession Number

P08249

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET RANVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMVCIIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL ATLTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVQSKET ECTYFSTPLL LGKKGLEKNL GIGKITPFEE KMIAEAIPEL KASIKKGEDF VKNMK