Recombinant mouse GalNAc Transferase 1/GALNT1 protein

Galnt1, also known as polypeptide N-acetylgalactosaminyltransferase 1, is a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. It catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Also, this protein is involved in the glycosylation of proteins essential for bone formation such as osteopontin and bone sialoprotein. Recombinant mouse Galnt1 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

60.5 kDa (528aa)

Additionnal Information

UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1, Protein-UDP acetylgalactosaminyltransferase 1, pp-GaNTase 1, Polypeptide N-acetylgalactosaminyltransferase 1, Polypeptide GalNAc transferase 1, Galnt1, GalNAc-T1, ATGP3718-10 µg, ATGP3718-20 µg, ATGP3718-50 µg, ATGP3718-100 µg, ATGP3718-250 µg, ATGP3718-500 µg, ATGP3718-1 mg, ATGP3718-010, ATGP3718-020, ATGP3718-050, ATGP3718-100, ATGP3718-250, ATGP3718-500, ATGP3718-01M

References & Citations

Li C., et al, (2016) Cancer Res. 76:1273-1283.; ; Huang MJ., et al, (2015) Oncotarget. 6:5650-5665

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_038842

Uniprot Accession Number

O08912

Species

Mouse

N Terminal Sequence

Ala

AA Sequence

ADPGLPAGDV LELVQKPHEG PGEMGKPVVI PKEDQEKMKE MFKINQFNLM ASEMIALNRS LPDVRLEGCK TKVYPDNLPT TSVVIVFHNE AWSTLLRTVH SVINRSPRHM IEEIVLVDDA SERDFLKRPL ESYVKKLKVP VHVIRMEQRS GLIRARLKGA AVSRGQVITF LDAHCECTAG WLEPLLARIK HDRRTVVCPI IDVISDDTFE YMAGSDMTYG GFNWKLNFRW YPVPQREMDR RKGDRTLPVR TPTMAGGLFS IDRDYFQEIG TYDAGMDIWG GENLEISFRI WQCGGTLEIV TCSHVGHVFR KATPYTFPGG TGQIINKNNR RLAEVWMDEF KNFFYIISPG VTKVDYGDIS SRLGLRRKLQ CKPFSWYLEN IYPDSQIPRH YFSLGEIRNV ETNQCLDNMA RKENEKVGIF NCHGMGGNQV FSYTANKEIR TDDLCLDVSK LNGPVTMLKC HHLKGNQLWE YDPVKLTLQH VNSNQCLDKA TEEDSQVPSI RDCTGSRSQQ WLLRNVTLPE IFHHHHHH