Recombinant human BTLA protein
BTLA, also known as B- and T-lymphocyte attenuator isoform 1, is an inhibitory molecule which belongs to the Ig superfamily. It is a type 1 transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. This protein is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1) . Also, it is a ligand for tumor necrosis factor (receptor) superfamily, member 14 (TNFRSF14), also known as herpes virus entry mediator (HVEM) . BTLA-HVEM complexes negatively regulate T-cell immune responses. Recombinant human BTLA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
B- and T-lymphocyte attenuator isoform 1, BTLA, BTLA1, CD272
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
15.7 kDa (136aa)
Additionnal Information
References & Citations
Watanabe N., et al, (2003) Nat. Immunol. 4:670-679. ; ; Gavrieli M., et al, (2003) Biochem. Biophys. Res. Commun. 312:1236-1243.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_861445.3
Uniprot Accession Number
Q7Z6A9
Species
Human
N Terminal Sequence
Ala
AA Sequence
KESCDVQ LYIKRQSEHS ILAGDPFELE CPVKYCANRP HVTWCKLNGT TCVKLEDRQT SWKEEKNISF FILHFEPVLP NDNGSYRCSA NFQSNLIESH STTLYVTDVK SASERPSKDE MASRPWLLYS
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