Recombinant human Nectin-3 protein

NECTIN3, also known as nectin-3 isoform 1, is a member of the nectin family. It is proposed to initiate cell-cell adhesion to promote cell attachment and to allow subsequent formation of JAM- and cadherin-based intercellular junctions. It has been shown to induce endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Subsequent Nectin-3 activity adds strength to the junction through trans-interaction with a variety of molecules. Recombinant human NECTIN3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nectin-3 isoform 1, NECTIN3, CD113, CDW113, NECTIN-3, PPR3, PRR3, PVRL3, PVRR3

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

39.1 kDa (355aa)

Additionnal Information

Nectin3, Nectin-3 isoform 1, NECTIN3, CD113, CDW113, NECTIN-3, PPR3, PRR3, PVRL3, PVRR3, ATGP3716-10 µg, ATGP3716-20 µg, ATGP3716-50 µg, ATGP3716-100 µg, ATGP3716-250 µg, ATGP3716-500 µg, ATGP3716-1 mg, ATGP3716-10, ATGP3716-20, ATGP3716-50, ATGP3716-100, ATGP3716-250, ATGP3716-500, ATGP3716-1

References & Citations

Rikitake, Y., et al. (2008) Cell. Mol. Life Sci. 65:253-263.; ; Miyoshi, J., et al. (2007) Am. J. Nephrol. 27:590-604.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Scientific Category

Immunology

NCBI Accession Number

NP_056295

Uniprot Accession Number

Q9NQS3

Species

Human

N Terminal Sequence

Gly58

AA Sequence

GPIIVEPHVT AVWGKNVSLK CLIEVNETIT QISWEKIHGK SSQTVAVHHP QYGFSVQGEY QGRVLFKNYS LNDATITLHN IGFSDSGKYI CKAVTFPLGN AQSSTTVTVL VEPTVSLIKG PDSLIDGGNE TVAAICIAAT GKPVAHIDWE GDLGEMESTT TSFPNETATI ISQYKLFPTR FARGRRITCV VKHPALEKDI RYSFILDIQY APEVSVTGYD GNWFVGRKGV NLKCNADANP PPFKSVWSRL DGQWPDGLLA SDNTLHFVHP LTFNYSGVYI CKVTNSLGQR SDQKVIYISD PPTTTTLQPT IQWHPSTADI EDLATEPKKL PFPLSTLATI KDDTIATLEH HHHHH