Recombinant human CD146/MCAM protein

MCAM, also known as cell surface glycoprotein MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. It is associated with various carcinomas such as tumor progression, metastasis and may be involved in embryonic neural development. This protein also plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Recombinant human MCAM protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Melanoma cell adhesion molecule, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, Gicerin, Melanoma-associated antigen MUC18, MUC18, S-endo 1 endothelial-associated antigen, CD146, MelCAM, METCAM, HEMCAM

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

61kDa (547aa)

Additionnal Information

MCAM, Cell surface glycoprotein MUC18, MCAM, CD146, MUC18, ATGP3715-10 µg, ATGP3715-20 µg, ATGP3715-50 µg, ATGP3715-100 µg, ATGP3715-250 µg, ATGP3715-500 µg, ATGP3715-1 mg, ATGP3715-10, ATGP3715-20, ATGP3715-50, ATGP3715-100, ATGP3715-250, ATGP3715-500, ATGP3715-1

References & Citations

Dagur PK., et al, (2014) Clin Immunol. 152:36-47.; ; Wragg JW., et al, (2016) Cancer Res. 76:2314-2326.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_006491

Uniprot Accession Number

P43121

Species

Human

N Terminal Sequence

Val24

AA Sequence

VPGEAEQPAP ELVEVEVGST ALLKCGLSQS QGNLSHVDWF SVHKEKRTLI FRVRQGQGQS EPGEYEQRLS LQDRGATLAL TQVTPQDERI FLCQGKRPRS QEYRIQLRVY KAPEEPNIQV NPLGIPVNSK EPEEVATCVG RNGYPIPQVI WYKNGRPLKE EKNRVHIQSS QTVESSGLYT LQSILKAQLV KEDKDAQFYC ELNYRLPSGN HMKESREVTV PVFYPTEKVW LEVEPVGMLK EGDRVEIRCL ADGNPPPHFS ISKQNPSTRE AEEETTNDNG VLVLEPARKE HSGRYECQGL DLDTMISLLS EPQELLVNYV SDVRVSPAAP ERQEGSSLTL TCEAESSQDL EFQWLREETG QVLERGPVLQ LHDLKREAGG GYRCVASVPS IPGLNRTQLV NVAIFGPPWM AFKERKVWVK ENMVLNLSCE ASGHPRPTIS WNVNGTASEQ DQDPQRVLST LNVLVTPELL ETGVECTASN DLGKNTSILF LELVNLTTLT PDSNTTTGLS TSTASPHTRA NSTSTERKLP EPESRGAAAL EHHHHHH