Recombinant human SHP-2/PTPN11 protein

PTPN11, also known as tyrosine-protein phosphatase non-receptor type 11 isoform 2, belongs to the protein-tyrosine phosphatase (PTP) family, non-receptor class 2 subfamily. It participates in signal transduction from the cell surface to the nucleus downstream of various receptor and cytosolic protein tyrosine kinases. This protein deficient cells have poor mobility because excessive phosphorylation of FAK and other proteins interferes with the rotation of cell attachment points in the focal adhesion complex. Recombinant human PTPN11, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Protein tyrosine phosphatase non-receptor type 11, NS1, Noonan syndrome 1, SH2 domain-containing protein tyrosine phosphatase 2, SHP-2, Protein-tyrosine phosphatase 1D, PTP-1D, Protein-tyrosine phosphatase 2C, PTP-2C, PTP2C, SHP2, BPTP3, SH-PTP2

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

53.9 kDa (469aa)

Additionnal Information

PTPN11, Tyrosine-protein phosphatase non-receptor type 11 isoform 2, PTPN11, BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2, ATGP3714-10 µg, ATGP3714-20 µg, ATGP3714-50 µg, ATGP3714-100 µg, ATGP3714-250 µg, ATGP3714-500 µg, ATGP3714-1 mg, ATGP3714-10, ATGP3714-20, ATGP3714-50, ATGP3714-100, ATGP3714-250, ATGP3714-500, ATGP3714-1

References & Citations

Clemmons, D.R., et al. (2005) Mol. Endocrinol. 19:1-11.; ; Maroun, C.R., et al. (2000) Mol. Cell. Biol. 20:8513-8525.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_542168.1

Uniprot Accession Number

Q06124

Species

Human

N Terminal Sequence

Ala

AA Sequence

MTSRRWF HPNITGVEAE NLLLTRGVDG SFLARPSKSN PGDFTLSVRR NGAVTHIKIQ NTGDYYDLYG GEKFATLAEL VQYYMEHHGQ LKEKNGDVIE LKYPLNCADP TSERWFHGHL SGKEAEKLLT EKGKHGSFLV RESQSHPGDF VLSVRTGDDK GESNDGKSKV THVMIRCQEL KYDVGGGERF DSLTDLVEHY KKNPMVETLG TVLQLKQPLN TTRINAAEIE SRVRELSKLA ETTDKVKQGF WEEFETLQQQ ECKLLYSRKE GQRQENKNKN RYKNILPFDH TRVVLHDGDP NEPVSDYINA NIIMPEFETK CNNSKPKKSY IATQGCLQNT VNDFWRMVFQ ENSRVIVMTT KEVERGKSKC VKYWPDEYAL KEYGVMRVRN VKESAAHDYT LRELKLSKVG QGNTERTVWQ YHFRTWPDHG VPSDPGGVLD FLEEVHHKQE SIMDAGPVVV HCR