Recombinant human Adenosine Kinase/ADK protein

Adenosine kinase, also known as ADK is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Recombinant human Adenosine kinase, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 30 pmol/min/ug and is defined as the amount of enzyme that convert 1.0 pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.

Product Name Alternative

ADK, AK, Adenosine kinase, Adenosine 5'-phosphotransferase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 30pmol/min/ug and is defined as the amount of enzyme that convert 1.0pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.

Molecular Weight

40.5 kDa (362aa) confirmed by MALDI-TOF

Additionnal Information

Adenosine Kinase, ADK, ADK, AK, ATGP3713-10 µg, ATGP3713-20 µg, ATGP3713-50 µg, ATGP3713-100 µg, ATGP3713-250 µg, ATGP3713-500 µg, ATGP3713-1 mg, ATGP3713-10, ATGP3713-20, ATGP3713-50, ATGP3713-100, ATGP3713-250, ATGP3713-500, ATGP3713-1

References & Citations

Chakraborty, A., et al. (2002) J. Biol. Chem. 277:47451-47451.; ; Sakowicz M., et al. (2001) Acta Biochim Pol. 48 (3) :745-54.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 1mM EDTA, 50 mM NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_006712

Uniprot Accession Number

P55263

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MRENILFGMG NPLLDISAVV DKDFLDKYSL KPNDQILAED KHKELFDELV KKFKVEYHAG GSTQNSIKVA QWMIQQPHKA ATFFGCIGID KFGEILKRKA AEAHVDAHYY EQNEQPTGTC AACITGDNRS LIANLAAANC YKKEKHLDLE KNWMLVEKAR VCYIAGFFLT VSPESVLKVA HHASENNRIF TLNLSAPFIS QFYKESLMKV MPYVDILFGN ETEAATFARE QGFETKDIKE IAKKTQALPK MNSKRQRIVI FTQGRDDTIM ATESEVTAFA VLDQDQKEII DTNGAGDAFV GGFLSQLVSD KPLTECIRAG HYAASIIIRR TGCTFPEKPD FH