Recombinant rat IL-6 protein
Il6, also known as interleukin 6, is an alpha-helical phosphorylated and glycosylated cytokine. This protein is important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism and cancer progression. Also, it can function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells. Recombinant rat Il6, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤0.1 ng/mL.
Product Name Alternative
Interleukin 6, Il6, Ifnb2, ILg6, Interferon beta 2
Expression System
Baculovirus
Antigen Species
Rat
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤0.1 ng/mL.
Molecular Weight
22.8 kDa (196aa)
Additionnal Information
References & Citations
Garbers C., et al, (2012) Cytokine Growth Factor Rev. 23:85-97.; ; Northemann W., et al, (1989) J. Biol. Chem. 264:16072-16082.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_036721
Uniprot Accession Number
P20607
Species
Rat
N Terminal Sequence
Ala
AA Sequence
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