Recombinant human TRAILR4/TNFRSF10D protein
TNFRSF10D, also known as tumor necrosis factor receptor superfamily member 10D, is a member of the TNF-receptor superfamily. This protein contains an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplasmic death domain. Also, it is can protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-mediated apoptosis. Recombinant human TNFRSF10D, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Measured by its ability to inhibit cytotoxicity using Jurkat human acute T cell leukemia cells. The ED50 range ≤10 ng/mL with Human TRAIL.
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its ability to inhibit cytotoxicity using Jurkat human acute T cell leukemia cells. The ED50 range ≤10 ng/mL with Human TRAIL.
Molecular Weight
43.8 kDa (395aa)
Additionnal Information
References & Citations
Degli-Esposti MA., et al, (1997) Immunity 7:813-820.; ; Marsters SA., et al, (1997) Curr. Biol. 7:1003-1006.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Apoptosis
NCBI Accession Number
NP_003831
Uniprot Accession Number
Q9UBN6
Species
Human
N Terminal Sequence
Ala56
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