Recombinant mouse AK2 protein
Ak2, also known as Adenylate kinase 2 mitochondrial isoform a, is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (Ak1, Ak2 and Ak3) are characterized in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. Ak2 is localized in the mitochondrial intermembrane space and may pay a role in apoptosis. Recombinant mouse Ak2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is > 40 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.
Product Name Alternative
Adenylate kinase 2 mitochondrial isoform a, Ak-2, D4Ertd220e, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, Adenylate monophosphate kinase
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 40unit/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.
Molecular Weight
29.0 kDa (263aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kohler C., et al. (1999) FEBS Lett. 447 (1) :10-2.; ; Lee Y. et al., (1998) J Biol chem. 123 (1) :47-54.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_001029138
Uniprot Accession Number
Q9WTP6
Species
Mouse
AA Sequence
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