Recombinant mouse RalA protein
Rala, also known as Ras-related protein Ral-A, is a member of the small GTPase superfamily, Ras family of proteins. Rala mediates a distinct downstream signaling pathway from Ras that facilitates cellular transformation. Rala is also thought to be involved in numerous signaling cascades, including regulation of the cytoskeleton, vesicle trafficking, and endocytosis. Recombinant mouse Rala protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ras-related protein Ral-A, 3010001O15Rik, AW322615, Ral, Rasl1, V-ral simian leukemia viral oncogene A, RAS like proto-oncogene A
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Purity
> 90% by SDS-PAGE
Molecular Weight
25.7 kDa (227aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Cascone I., et al. (2008) EMBO J. 27 (18) :2375-2387.; ; Balasubramanian N., et al. (2010) Curr. Biol. 20 (1) :75-79.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 30% glycerol, 0.1M NaCl, 0.1mM PMSF
Scientific Category
Signal Transduction
NCBI Accession Number
NP_062364
Uniprot Accession Number
P63321
Species
Mouse
AA Sequence
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