Recombinant mouse Glutaredoxin 1/GLRX1 protein
Glrx, also known as glutaredoxin-1, is member of the thiol-disulfide oxidoreductase family. Glutraredoxin catalyzes the reversible reduction of protein-glutathionyl mixed disulfides to free sulfhydryl groups though a monothiol mechanism. Mammalian Glutaredoxin is known to have two isoforms, GRX1 and GRX2. GRX1 is a cytosolic protein, whereas GRX2 is localized both in the mitochondria and nucleus. Glrx may be involved in a various cellular events such as signal transduction, stress response, and metabolic regulation by regulating the redox status of cellular proteins. Recombinant mouse Glrx protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Glutaredoxin-1, C86710, D13Wsu156e, Glrx1, Grx1, Ttase, Thioltransferase-1
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by BCA assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
14.3 kDa (130aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Reynaert NL., et al. (2006) Proc Natl Acad Sci U S A. 103 (35) :13086-91.; ; Kanda M., et al. (2006) J Biol Chem. 281 (39) :28518-28.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM MES buffer (pH 5.0) containing 20% glycerol, 0.1M NaCl
Scientific Category
Redox Proteins
NCBI Accession Number
NP_444338
Uniprot Accession Number
Q9QUH0
Species
Mouse
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAQEFVN CKIQSGKVVV FIKPTCPYCR KTQEILSQLP FKQGLLEFVD ITATNNTSAI QDYLQQLTGA RTVPRVFIGK DCIGGCSDLI SMQQTGELMT RLKQIGALQL
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