Recombinant mouse Glutaredoxin 1/GLRX1 protein

Glrx, also known as glutaredoxin-1, is member of the thiol-disulfide oxidoreductase family. Glutraredoxin catalyzes the reversible reduction of protein-glutathionyl mixed disulfides to free sulfhydryl groups though a monothiol mechanism. Mammalian Glutaredoxin is known to have two isoforms, GRX1 and GRX2. GRX1 is a cytosolic protein, whereas GRX2 is localized both in the mitochondria and nucleus. Glrx may be involved in a various cellular events such as signal transduction, stress response, and metabolic regulation by regulating the redox status of cellular proteins. Recombinant mouse Glrx protein was expressed in E. coli and purified by using conventional chromatography techniques.