Recombinant human CD58/LFA-3 protein

CD58, also known as lymphocyte function-associated antigen 3 isoform 1, is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. It is an important regulator of immune cell function which occurs as both surface-associated and soluble forms. It is associated with increased risk for multiple sclerosis. Recombinant human CD58, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Lymphocyte function-associated antigen 3 isoform 1, CD58, ag3, LFA-3, LFA3

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.5 kDa (196aa)

Additionnal Information

CD58, LFA3, Lymphocyte function-associated antigen 3 isoform 1, CD58, ag3, LFA-3, LFA3, ATGP3693-10 µg, ATGP3693-20 µg, ATGP3693-50 µg, ATGP3693-100 µg, ATGP3693-250 µg, ATGP3693-500 µg, ATGP3693-1 mg, ATGP3693-10, ATGP3693-20, ATGP3693-50, ATGP3693-100, ATGP3693-250, ATGP3693-500, ATGP3693-1

References & Citations

Abdul Razak FR., et al. (2016) Genes Immun. 17:363-366.; ; Kirby AC., et al. (2000) Cell Adhes Commun. 7:453-464.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_001770

Uniprot Accession Number

P19256

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPFSQQIYG VVYGNVTFHV PSNVPLKEVL WKKQKDKVAE LENSEFRAFS SFKNRVYLDT VSGSLTIYNL TSSDEDEYEM ESPNITDTMK FFLYVLESLP SPTLTCALTN GSIEVQCMIP EHYNSHRGLI MYSWDCPMEQ CKRNSTSIYF KMENDLPQKI QCTLSNPLFN TTSSIILTTC IPSSGHSRHR HHHHHH