Recombinant mouse Adenosine Kinase/ADK protein

Adk, also known as Adenosine kinase, is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Recombinant mouse Adk, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 100 pmol/min/ and is defined as the amount of enzyme that convert 1.0 pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.

Product Name Alternative

ADK, AK, adenosine kinase, Adenosine 5'-phosphotransferase

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 100pmol/min/ and is defined as the amount of enzyme that convert 1.0pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.

Molecular Weight

42.5 kDa (384aa) confirmed by MALDI-TOF

Additionnal Information

Adk, Adenosine kinase, 2310026J05Rik, 5033405D03Rik, AI255373, AI987814, Ak, ATGP3692-10 µg, ATGP3692-20 µg, ATGP3692-50 µg, ATGP3692-100 µg, ATGP3692-250 µg, ATGP3692-500 µg, ATGP3692-1 mg, ATGP3692-10, ATGP3692-20, ATGP3692-50, ATGP3692-100, ATGP3692-250, ATGP3692-500, ATGP3692-1

References & Citations

Chakraborty A., et al. (2002) J Biol Chem. 277 (49: 47451-60.; ; Sakowicz M., et al. (2001) Acta Biochim Pol. 48 (3) :745-54.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 50 mM NaCl, 1mM EDTA

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_598840

Uniprot Accession Number

P55264

Species

Mouse

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMAAADEP KPKKLKVEAP QALSENVLFG MGNPLLDISA VVDKDFLDKY SLKPNDQILA EDKHKELFDE LVKKFKVEYH AGGSTQNSMK VAQWLIQEPH KAATFFGCIG IDKFGEILKR KAADAHVDAH YYEQNEQPTG TCAACITGGN RSLVANLAAA NCYKKEKHLD LERNWVLVEK ARVYYIAGFF LTVSPESVLK VARYAAENNR VFTLNLSAPF ISQFFKEALM DVMPYVDILF GNETEAATFA REQGFETKDI KEIAKKAQAL PKVNSKRQRT VIFTQGRDDT IVAAENDVTA FPVLDQNQEE IIDTNGAGDA FVGGFLSQLV SDKPLTECIR AGHYAASVII RRTGCTFPEK PDFH