Recombinant mouse Enolase 1/ENO1 protein

Eno1 also known as Alpha-Enolase, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of Eno1 is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma. Recombinant mouse Eno1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is > 6,000 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0 pmole of 2-phosphoglycerate to phosphoenolpyruvate per minute at pH 6.5 at 37C in a couple system with PK and LDH.

Product Name Alternative

2-phospho-D-glycerate hydro-lyase, Enolase 1, Non-neural enolase, NNE, c-Myc promoter binding protein

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 6,000pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of 2-phosphoglycerate to phosphoenolpyruvate per minute at pH 6.5 at 37C in a couple system with PK and LDH.

Molecular Weight

49.5 kDa (457aa) confirmed by MALDI-TOF

Additionnal Information

Enolase1, Non-neural enolase, Alpha-Enolase, 0610008I15, AL022784, Eno-1, MBP-1, ATGP3691-10 µg, ATGP3691-20 µg, ATGP3691-50 µg, ATGP3691-100 µg, ATGP3691-250 µg, ATGP3691-500 µg, ATGP3691-1 mg, ATGP3691-10, ATGP3691-20, ATGP3691-50, ATGP3691-100, ATGP3691-250, ATGP3691-500, ATGP3691-1

References & Citations

Plow EF., et al. (2009) Blood. 113 (22) :5371-2. ; ; Bartholomeusz C., et al. (2008) Cancer Res. 68 (22) :9302-10.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 40% glycerol, 0.1M NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_075608

Uniprot Accession Number

P17182

Species

Mouse

AA Sequence

MSILRIH AREIFDSRGN PTVEVDLYTA KGLFRAAVPS GASTGIYEAL ELRDNDKTRF MGKGVSQAVE HINKTIAPAL VSKKVNVVEQ EKIDKLMIEM DGTENKSKFG ANAILGVSLA VCKAGAVEKG VPLYRHIADL AGNPEVILPV PAFNVINGGS HAGNKLAMQE FMILPVGASS FREAMRIGAE VYHNLKNVIK EKYGKDATNV GDEGGFAPNI LENKEALELL KTAIAKAGYT DQVVIGMDVA ASEFYRSGKY DLDFKSPDDP SRYITPDQLA DLYKSFVQNY PVVSIEDPFD QDDWGAWQKF TASAGIQVVG DDLTVTNPKR IAKAASEKSC NCLLLKVNQI GSVTESLQAC KLAQSNGWGV MVSHRSGETE DTFIADLVVG LCTGQIKTGA PCRSERLAKY NQILRIEEEL GSKAKFAGRS FRNPLAK