Recombinant mouse Enolase 1/ENO1 protein
Eno1 also known as Alpha-Enolase, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of Eno1 is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma. Recombinant mouse Eno1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Specifications
Product Name Alternative
2-phospho-D-glycerate hydro-lyase, Enolase 1, Non-neural enolase, NNE, c-Myc promoter binding protein
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Purity
> 90% by SDS-PAGE
Bioactivity
Molecular Weight
49.5 kDa (457aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Plow EF., et al. (2009) Blood. 113 (22) :5371-2. ; ; Bartholomeusz C., et al. (2008) Cancer Res. 68 (22) :9302-10.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 40% glycerol, 0.1M NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_075608
Uniprot Accession Number
P17182
Species
Mouse
AA Sequence
Frequently Asked Questions
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